abc horseradish peroxidase labelled vectastain elite abc reagent  (Vector Laboratories)

Journal: Environmental Health Perspectives

Article Title: Prolonged, Low-Level Exposure to the Marine Toxin, Domoic Acid, and Measures of Neurotoxicity in Nonhuman Primates

doi: 10.1289/EHP10923

Figure Lengend Snippet: Hippocampal Iba-1 immunoreactivity. Representative images of immunostaining of 10% formalin-fixed, paraffin-embedded, 10 - μ m sections for Iba- 1 + (1:2,000; Wako Chemicals) microglia in the hippocampus of female Macaca fascicularis following prolonged exposed to domoic acid ( 0.15 -mg / kg BW per day ) or vehicle (5% sucrose). Images represent the hippocampus ( scale bar : 2,000 μ m ) and specific hippocampal regions, including the dentate gyrus (scale bars: 200 and 100 μ m ) and the CA3 and CA1 pyramidal cell layers ( scale bar : 100 μ m ). Immunoreactivity was visualized with Vectastain Elite and shows as darker process-bearing cells within the image. Sections were counterstained with cresyl violet (CV). Note: CA, cornu ammonis area; Iba- 1 , ionized calcium binding adaptor molecule 1.

Article Snippet: Antigen–antibody complexes were visualized with a Vectastain Elite ABC R.T.U. label (Vector Laboratories,) and 3,3′-diaminobenzidine (Dakocytomation Corp.).

Techniques: Immunostaining, Formalin-fixed Paraffin-Embedded, Binding Assay

Journal: Environmental Health Perspectives

Article Title: Prolonged, Low-Level Exposure to the Marine Toxin, Domoic Acid, and Measures of Neurotoxicity in Nonhuman Primates

doi: 10.1289/EHP10923

Figure Lengend Snippet: Differences in glial morphology in the thalamus. Representative images of staining of 10% formalin-fixed, paraffin-embedded, 10 - μ m sections of the thalamus for cresyl violet (CV) and immunohistochemistry ionized calcium binding adaptor molecule 1 (Iba-1; 1:2,000; Wako Chemicals) microglia; and glial fibrillary acidic protein (GFAP; 1:7,000, Dakocytomation) astrocytes from female Macaca fascicularis following prolonged exposed to domoic acid ( 0.15 -mg / kg BW per day ) or vehicle (5% sucrose). Immunoreactivity was visualized with Vectastain Elite and sections were counterstained with CV. CV staining showed no evidence of differences in the distribution of Nissl substance. Representative immunoreactive cells for Iba-1 or GFAP display as darker stained cells. Scale bar : 100 μ m .

Article Snippet: Antigen–antibody complexes were visualized with a Vectastain Elite ABC R.T.U. label (Vector Laboratories,) and 3,3′-diaminobenzidine (Dakocytomation Corp.).

Techniques: Staining, Formalin-fixed Paraffin-Embedded, Immunohistochemistry, Binding Assay

Journal: Environmental Health Perspectives

Article Title: Prolonged, Low-Level Exposure to the Marine Toxin, Domoic Acid, and Measures of Neurotoxicity in Nonhuman Primates

doi: 10.1289/EHP10923

Figure Lengend Snippet: Focal sites of microglia reactivity. Representative images of Iba-1 immunopositive microglia in 10% formalin-fixed, paraffin-embedded, 10 - μ m sections at focal sites of reactivity in the thalamus, fornix, fimbria, internal capsule, and nucleus accumbens of female Macaca fascicularis following prolonged exposed to domoic acid ( 0.15 -mg / kg BW per day ) or vehicle (5% sucrose). Representative image of the nucleus accumbens in vehicle control is included for comparison. Representative images for the vehicle control fornix, fimbria, internal capsule, and thalamus are provided in and . Numbers correspond to Animal Numbers in Table S1. A15244 and A16106 were in the 0.15 -mg / kg BW per day group; A15249, A16107, and A16106 were in the 0.075 -mg / kg BW per day group; and A15428 was in the control group. Microglia were immunostained with antibody to Iba-1 (1:2,000; Wako Chemicals) followed by IgG antibody, visualized with Vectastain Elite (brown), and counterstained with cresyl violet (CV). Scale bar : 100 μ m .

Article Snippet: Antigen–antibody complexes were visualized with a Vectastain Elite ABC R.T.U. label (Vector Laboratories,) and 3,3′-diaminobenzidine (Dakocytomation Corp.).

Techniques: Formalin-fixed Paraffin-Embedded, Control, Comparison